This comprehensive textbook covers a wide range of instrumental analytical techniques used in chemistry, pharmaceuticals, biotechnology, and environmental science. It provides a detailed explanation of spectroscopic, chromatographic, and electrophoretic methods, along with their theoretical background, instrumentation, and applications.
The book begins with UV-Visible Spectroscopy, discussing electronic transitions, spectral shifts, and Beer-Lambert’s law, followed by Fluorimetry, explaining fluorescence principles, quenching effects, and instrumentation. IR Spectroscopy is detailed with vibrational modes, sample handling, and detector technologies.
Flame Photometry and Atomic Absorption Spectroscopy are described, focusing on flame excitation, interferences, and practical applications. The Nepheloturbidometry section introduces light scattering principles for analyzing suspensions and colloids.
A strong emphasis is placed on Chromatographic Techniques, covering Adsorption and Partition Chromatography, Thin Layer Chromatography (TLC), Paper Chromatography, Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), Ion Exchange Chromatography, Gel Chromatography, and Affinity Chromatography. Each technique includes methodology, instrumentation, advantages, and applications.
The book also explores Electrophoresis, detailing paper, gel, and capillary techniques for separating biomolecules. Advanced topics such as Derivatization in GC, Temperature Programming, and Ion Exchange Mechanisms provide deeper insights into modern analytical methods.
Designed for students, researchers, and professionals, this book offers theoretical clarity, practical guidance, and real-world applications in pharmaceutical analysis, environmental testing, food safety, and clinical research. It is an essential resource for mastering instrumental methods of analysis.